![]() ![]() After cooling, add a few crystals of sodium carbonate, and after 24 hr, filter and add water to 1 L. Dissolve 200 g of anhydrous sodium carbonate in 800 mL of water and bring to a boil. Can be opened daily, but to store, keep closed in a refrigerator up to two weeks. In a 100-mL volumetric flask, dissolve 0.500 g of dry gallic acid in10 mL of ethanol and dilute to volume with water. Solutions containing the FC reagent must be disposed of as hazardous waste. Singleton and Rossi AJEV 1965, 16: 144-158, describe the preparation of the reagent from sodium tungstate, sodium molybdate, lithium sulfate, bromine, and some acids. This reduces waste and disposal volume.įolin Ciocalteu Reagent. This is usually purchased as the 2N reagent available from Sigma (F9252) or from Fisher Scientific (ICN19518690), and presumably others. If you cannot reproducibly measure such small volumes, try to reduce the volumes to the smallest you can. Total Phenol Analysis: Automation and Comparison with Manual Methods. American Journal of Enology and Viticulture 1977, 28: 49-55 only the volumes have been reduced. It is based on the method reported in Slinkard, K. Good micro pipets must be used for reproducibility. It uses the minimum volume of reagents and almost eliminates wasted reagent. The procedure is also used for analysis of total phenol in tea. This method is used routinely in our lab to measure total phenol. ![]()
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